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汪志军

副研究员

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学术经历

  • 2007年起任职于ebet微生物代谢教育部重点实验室。2011-2013,加州大学伯克利分校,唐氏学者。

研究方向

天然产物合成机器装配的候选研究主题

1.常用的抗生素如红霉素、青霉素的天然产物的合成机器。

2.洛伐他丁类的合成机器:

洛伐他丁也是天然产物,是新型降血脂药物。用于治疗高胆固醇血症和混合型高脂血症,对动脉粥样硬化和冠心病有防治作用。由洛伐他丁衍生的他丁类药物,同时也是当前癌症治疗药物研究的焦点之一。

冷冻电镜技术解析蛋白结构

1.蛋白分子机器的表达和纯化
2.液态乙烷中蛋白质冷冻样品的制备
3.冷冻电镜操作与观察
4.计算解析蛋白三维结构模型

代表论著

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    Pu T, Liang J, Mei Z, Yang Y, Wang J, Zhang W, Liang W, Zhou X, Deng Z*, Wang Z*. Phosphorothioated DNA is shielded from oxidative damage. Applied and Environmental Microbiology. 2019 Feb 8. pii: AEM.00104-19. doi: 10.1128/AEM.00104-19. [Epub ahead of print]

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    Wu H, Liang J, Gou L, Wu Q, Liang W, Zhou X, Bruce IJ, Deng Z*, Wang Z*. Recycling of Overactivated Acyls by a Type II Thioesterase during Calcimycin Biosynthesis in Streptomyces chartreusis NRRL 3882.  Applied and Environmental Microbiology.  2018 May 31;84(12). e00587-18.

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    Wu H, Liang J, Wang J, Liang W, Gou L, Wu Q, Zhou X, Bruce IJ, Deng Z*, Wang Z*. Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882. Applied and Environmental Microbiology.  2018 May 31;84(12). e00586-18.

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    Zhao Y, Li L, Zheng G, Jiang W, Deng Z, Wang Z*, Lu Y*. CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces. Biotechnology Journal.  2018 Sep;13(9): e1800121.

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    Dai D, Pu T, Liang J, Wang Z*,Tang A*.  Regulation of dndB Gene Expression in Streptomyces lividans. Frontiers in Microbiology.  2018 Oct 8; 9:2387.

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    Yang Y, Xu G, Liang J, He Y, Xiong L, Li H, Bartlett D, Deng Z, Wang Z*, Xiao X*. DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function. Scientific Reports.  2017 Jun 14;7(1):3516.

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    Gou L, Han T*, Wang X, Ge J, Liu W, Hu F, Wang Z*. A Novel TetR Family Transcriptional Regulator, CalR3, Negatively Controls Calcimycin Biosynthesis in Streptomyces chartreusis NRRL 3882.  Frontiers in Microbiology.  2017 Nov 29; 8:2371.

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    Dai D, Du A, Xiong K, Pu T, Zhou X, Deng Z, Liang J*, He X*, Wang Z*.  DNA Phosphorothioate Modification Plays a Role in Peroxides Resistance in Streptomyces lividans. Frontiers in Microbiology.  2016 Aug 31; 7:1380.

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    Li F, Liang J, Wang W, Zhou X, Deng Z*, Wang Z*. Two nucleoside receptors from Streptomyces coelicolor: expression of the genes and characterization of the recombinant proteins. Protein Expression and Purification.  2015 May; 109:40-6.

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    Li F, Liang J, Wang W, Zhou X, Deng Z*, Wang Z*.  Analysis of Streptomyces coelicolor membrane proteome using two-dimensional native/native and native/sodium dodecyl sulfate gel electrophoresis. Analytical biochemistry.  2014 Aug 18; 465C:148-155.